Suppression of NF-κB-mediatedβ-defensin gene expression in the mammalian airway by theBordetellatype III secretion system.

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  • Additional Information
    • Abstract:
      Expression of innate immune genes such asβ-defensins is induced in airway epithelium by bacterial components via activation of NF-κB. We show here that live Gram-negative bacteria can similarly stimulate this pathway, resulting in upregulation of theβ-defensin tracheal antimicrobial peptide (TAP) in primary cultures of bovine tracheal epithelial cells (TECs), by a Toll-like receptor 4 (TLR4)-mediated pathway. The Gram-negative airway pathogenBordetella bronchisepticapossesses a type III secretion system previously suggested to inhibit the nuclear translocation of NF-κB in a cell line by immunohistochemistry. We therefore hypothesized that this pathogen might interfere in the innate immune response of the epithelium. Exposure of TECs to wild-typeB. bronchisepticasuppressed the activation of NF-κB and the subsequent induction of TAP mRNA levels, whereas a type III secretion-defective strain did not. These results suggest a mechanism for bacterial evasion of the innate immune response in the airway, which could allow for the observed persistent colonization of this pathogen. [ABSTRACT FROM AUTHOR]
    • Abstract:
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    • Author Affiliations:
      1Immunobiology Center, Mount Sinai School of Medicine, New York, USA
      2Department of Oral Biology, UMDNJ — New Jersey Dental School, Newark, NJ, USA
      3UMDNJ — Graduate School of Biomedical Sciences, Newark, NJ, USA
      4Department Of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, PA, USA
    • ISSN:
      1462-5814
    • Accession Number:
      10.1111/j.1462-5822.2004.00473.x
    • Accession Number:
      16370808
  • Citations
    • ABNT:
      LEGARDA, D. et al. Suppression of NF-κB-mediatedβ-defensin gene expression in the mammalian airway by theBordetellatype III secretion system. Cellular Microbiology, [s. l.], v. 7, n. 4, p. 489–497, 2005. Disponível em: . Acesso em: 12 nov. 2019.
    • AMA:
      Legarda D, Klein-Patel ME, Sunghan Yim, Yuk MH, Diamond G. Suppression of NF-κB-mediatedβ-defensin gene expression in the mammalian airway by theBordetellatype III secretion system. Cellular Microbiology. 2005;7(4):489-497. doi:10.1111/j.1462-5822.2004.00473.x.
    • APA:
      Legarda, D., Klein-Patel, M. E., Sunghan Yim, Yuk, M. H., & Diamond, G. (2005). Suppression of NF-κB-mediatedβ-defensin gene expression in the mammalian airway by theBordetellatype III secretion system. Cellular Microbiology, 7(4), 489–497. https://doi.org/10.1111/j.1462-5822.2004.00473.x
    • Chicago/Turabian: Author-Date:
      Legarda, Diana, Marcia E. Klein-Patel, Sunghan Yim, Ming H. Yuk, and Gill Diamond. 2005. “Suppression of NF-ΚB-Mediatedβ-Defensin Gene Expression in the Mammalian Airway by TheBordetellatype III Secretion System.” Cellular Microbiology 7 (4): 489–97. doi:10.1111/j.1462-5822.2004.00473.x.
    • Harvard:
      Legarda, D. et al. (2005) ‘Suppression of NF-κB-mediatedβ-defensin gene expression in the mammalian airway by theBordetellatype III secretion system’, Cellular Microbiology, 7(4), pp. 489–497. doi: 10.1111/j.1462-5822.2004.00473.x.
    • Harvard: Australian:
      Legarda, D, Klein-Patel, ME, Sunghan Yim, Yuk, MH & Diamond, G 2005, ‘Suppression of NF-κB-mediatedβ-defensin gene expression in the mammalian airway by theBordetellatype III secretion system’, Cellular Microbiology, vol. 7, no. 4, pp. 489–497, viewed 12 November 2019, .
    • MLA:
      Legarda, Diana, et al. “Suppression of NF-ΚB-Mediatedβ-Defensin Gene Expression in the Mammalian Airway by TheBordetellatype III Secretion System.” Cellular Microbiology, vol. 7, no. 4, Apr. 2005, pp. 489–497. EBSCOhost, doi:10.1111/j.1462-5822.2004.00473.x.
    • Chicago/Turabian: Humanities:
      Legarda, Diana, Marcia E. Klein-Patel, Sunghan Yim, Ming H. Yuk, and Gill Diamond. “Suppression of NF-ΚB-Mediatedβ-Defensin Gene Expression in the Mammalian Airway by TheBordetellatype III Secretion System.” Cellular Microbiology 7, no. 4 (April 2005): 489–97. doi:10.1111/j.1462-5822.2004.00473.x.
    • Vancouver/ICMJE:
      Legarda D, Klein-Patel ME, Sunghan Yim, Yuk MH, Diamond G. Suppression of NF-κB-mediatedβ-defensin gene expression in the mammalian airway by theBordetellatype III secretion system. Cellular Microbiology [Internet]. 2005 Apr [cited 2019 Nov 12];7(4):489–97. Available from: http://widgets.ebscohost.com/prod/customlink/proxify/proxify.php?count=1&encode=0&proxy=&find_1=&replace_1=&target=http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=a9h&AN=16370808&authtype=sso&custid=s5834912