Gene expression patterns associated with blood-feeding in the malaria mosquito Anopheles gambiae.

Item request has been placed! ×
Item request cannot be made. ×
  Processing Request
  • Additional Information
    • NAICS/Industry Codes:
      541710 Research and development in the physical, engineering and life sciences
    • Abstract:
      Background: Blood feeding, or hematophagy, is a behavior exhibited by female mosquitoes required both for reproduction and for transmission of pathogens. We determined the expression patterns of 3,068 ESTs, representing ~2,000 unique gene transcripts using cDNA microarrays in adult female Anopheles gambiae at selected times during the first two days following blood ingestion, at 5 and 30 min during a 40 minute blood meal and at 0, 1, 3, 5, 12, 16, 24 and 48 hours after completion of the blood meal and compared their expression to transcript levels in mosquitoes with access only to a sugar solution. Results: In blood-fed mosquitoes, 413 unique transcripts, approximately 25% of the total, were expressed at least two-fold above or below their levels in the sugar-fed mosquitoes, at one or more time points. These differentially expressed gene products were clustered using k-means clustering into Early Genes, Middle Genes, and Late Genes, containing 144, 130, and 139 unique transcripts, respectively. Several genes from each group were analyzed by quantitative real-time PCR in order to validate the microarray results. Conclusion: The expression patterns and annotation of the genes in these three groups (Early, Middle, and Late genes) are discussed in the context of female mosquitoes' physiological responses to blood feeding, including blood digestion, peritrophic matrix formation, egg development, and immunity. [ABSTRACT FROM AUTHOR]
    • Abstract:
      Copyright of BMC Genomics is the property of BioMed Central and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
    • Author Affiliations:
      1Center for Tropical Disease Research and Training, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA
      2Department of Tropical Medicine, Tulane University, New Orleans, LA 70112, USA
      3Department of Zoology, University of Toronto, Toronto, ON M5S 3G5, Canada
    • ISSN:
      1471-2164
    • Accession Number:
      10.1186/1471-2164-6-5
    • Accession Number:
      28858446
  • Citations
    • ABNT:
      DANA, A. N. et al. Gene expression patterns associated with blood-feeding in the malaria mosquito Anopheles gambiae. BMC Genomics, [s. l.], v. 6, p. 1–24, 2005. Disponível em: . Acesso em: 20 nov. 2019.
    • AMA:
      Dana AN, Hong YS, Kern MK, et al. Gene expression patterns associated with blood-feeding in the malaria mosquito Anopheles gambiae. BMC Genomics. 2005;6:1-24. doi:10.1186/1471-2164-6-5.
    • APA:
      Dana, A. N., Hong, Y. S., Kern, M. K., Hillenmeyer, M. E., Harker, B. W., Lobo, N. F., … Collins, F. H. (2005). Gene expression patterns associated with blood-feeding in the malaria mosquito Anopheles gambiae. BMC Genomics, 6, 1–24. https://doi.org/10.1186/1471-2164-6-5
    • Chicago/Turabian: Author-Date:
      Dana, Ali N., Young S. Hong, Marcia K. Kern, Maureen E. Hillenmeyer, Brent W. Harker, Neil F. Lobo, James R. Hogan, Patricia Romans, and Frank H. Collins. 2005. “Gene Expression Patterns Associated with Blood-Feeding in the Malaria Mosquito Anopheles Gambiae.” BMC Genomics 6 (January): 1–24. doi:10.1186/1471-2164-6-5.
    • Harvard:
      Dana, A. N. et al. (2005) ‘Gene expression patterns associated with blood-feeding in the malaria mosquito Anopheles gambiae’, BMC Genomics, 6, pp. 1–24. doi: 10.1186/1471-2164-6-5.
    • Harvard: Australian:
      Dana, AN, Hong, YS, Kern, MK, Hillenmeyer, ME, Harker, BW, Lobo, NF, Hogan, JR, Romans, P & Collins, FH 2005, ‘Gene expression patterns associated with blood-feeding in the malaria mosquito Anopheles gambiae’, BMC Genomics, vol. 6, pp. 1–24, viewed 20 November 2019, .
    • MLA:
      Dana, Ali N., et al. “Gene Expression Patterns Associated with Blood-Feeding in the Malaria Mosquito Anopheles Gambiae.” BMC Genomics, vol. 6, Jan. 2005, pp. 1–24. EBSCOhost, doi:10.1186/1471-2164-6-5.
    • Chicago/Turabian: Humanities:
      Dana, Ali N., Young S. Hong, Marcia K. Kern, Maureen E. Hillenmeyer, Brent W. Harker, Neil F. Lobo, James R. Hogan, Patricia Romans, and Frank H. Collins. “Gene Expression Patterns Associated with Blood-Feeding in the Malaria Mosquito Anopheles Gambiae.” BMC Genomics 6 (January 2005): 1–24. doi:10.1186/1471-2164-6-5.
    • Vancouver/ICMJE:
      Dana AN, Hong YS, Kern MK, Hillenmeyer ME, Harker BW, Lobo NF, et al. Gene expression patterns associated with blood-feeding in the malaria mosquito Anopheles gambiae. BMC Genomics [Internet]. 2005 Jan [cited 2019 Nov 20];6:1–24. Available from: http://widgets.ebscohost.com/prod/customlink/proxify/proxify.php?count=1&encode=0&proxy=&find_1=&replace_1=&target=http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=a9h&AN=28858446&authtype=sso&custid=s5834912