Development of an RP-UHPLC-PDA method for quantification of free gossypol in cottonseed cake and fungal-treated cottonseed cake.

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  • Additional Information
    • Publication Year:
      2018
    • Author-Supplied Keywords:
      Acetones
      Agriculture
      Biology and life sciences
      Chemical compounds
      Chemistry
      Chromatographic techniques
      Cotton
      Detoxification
      Elution
      Eukaryota
      Flowering plants
      Fungi
      High performance liquid chromatography
      Liquid chromatography
      Livestock
      Medicine and health sciences
      Organic chemistry
      Organic compounds
      Organisms
      Pathology and laboratory medicine
      Physical sciences
      Plants
      Research and analysis methods
      Research Article
      Separation processes
      Toxicology
    • Abstract:
      Cottonseed cake biomass, which is a residue of oil extraction, is potentially appropriate for use as animal feed, given the high mineral, fibre and protein content. The presence of free gossypol, however, a toxic pigment in the glands of the cotton plant, limits use of this biomass for monogastric livestock. A promising method to detoxify cottonseed cake relies on fermentation by fungi, which can eliminate up to 100% of gossypol. In order to quantify trace levels of free gossypol in different cotton materials, including cottonseed cake treated with macrofungi, a simple and rapid chromatographic detection method was developed and validated. Under optimized conditions, extraction was performed using 70% acetone. The extract was then analysed by Ultra High-Performance Liquid Chromatography (UHPLC), with gradient elution on a C18 reverse phase column KINETEX® (100 x 2.10 mm, 2.6 μm). Methanol-0.1% TFA aqueous solution was employed as mobile phase and PDA detection conducted at 254 nm. The optimized method was validated by analysis of specificity, linearity and range, limit of detection, limit of quantification, precision and accuracy. Detection and quantification limits were observed at 0.2 and 0.5 μg/mL, respectively. With good reproducibility, with precision (RSD)<10% and recovery greater than 94%, the developed assay was appropriate for quantification of low quantities of free gossypol. The validated method was successfully applied to determine trace levels of free gossypol cottonseed treated with a macrofungus. [ABSTRACT FROM AUTHOR]
    • Abstract:
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    • ISSN:
      19326203
    • Accession Number:
      129741097
  • Citations
    • ABNT:
      CONCEIÇÃO, A. A. et al. Development of an RP-UHPLC-PDA method for quantification of free gossypol in cottonseed cake and fungal-treated cottonseed cake. PLoS ONE, [s. l.], v. 13, n. 5, p. 1, 2018. Disponível em: . Acesso em: 24 ago. 2019.
    • AMA:
      Conceição AA, Soares Neto CB, Ribeiro JA de A, Siqueira FG de, Miller RNG, Mendonça S. Development of an RP-UHPLC-PDA method for quantification of free gossypol in cottonseed cake and fungal-treated cottonseed cake. PLoS ONE. 2018;13(5):1. http://ezproxy.parker.edu/login?url={targeturl}. Accessed August 24, 2019.
    • APA:
      Conceição, A. A., Soares Neto, C. B., Ribeiro, J. A. de A., Siqueira, F. G. de, Miller, R. N. G., & Mendonça, S. (2018). Development of an RP-UHPLC-PDA method for quantification of free gossypol in cottonseed cake and fungal-treated cottonseed cake. PLoS ONE, 13(5), 1. Retrieved from http://ezproxy.parker.edu/login?url={targeturl}
    • Chicago/Turabian: Author-Date:
      Conceição, Aparecido Almeida, Clemente Batista Soares Neto, José Antônio de Aquino Ribeiro, Felix Gonçalves de Siqueira, Robert Neil Gerard Miller, and Simone Mendonça. 2018. “Development of an RP-UHPLC-PDA Method for Quantification of Free Gossypol in Cottonseed Cake and Fungal-Treated Cottonseed Cake.” PLoS ONE 13 (5): 1. http://ezproxy.parker.edu/login?url={targeturl}.
    • Harvard:
      Conceição, A. A. et al. (2018) ‘Development of an RP-UHPLC-PDA method for quantification of free gossypol in cottonseed cake and fungal-treated cottonseed cake’, PLoS ONE, 13(5), p. 1. Available at: http://ezproxy.parker.edu/login?url={targeturl} (Accessed: 24 August 2019).
    • Harvard: Australian:
      Conceição, AA, Soares Neto, CB, Ribeiro, JA de A, Siqueira, FG de, Miller, RNG & Mendonça, S 2018, ‘Development of an RP-UHPLC-PDA method for quantification of free gossypol in cottonseed cake and fungal-treated cottonseed cake’, PLoS ONE, vol. 13, no. 5, p. 1, viewed 24 August 2019, .
    • MLA:
      Conceição, Aparecido Almeida, et al. “Development of an RP-UHPLC-PDA Method for Quantification of Free Gossypol in Cottonseed Cake and Fungal-Treated Cottonseed Cake.” PLoS ONE, vol. 13, no. 5, May 2018, p. 1. EBSCOhost, ezproxy.parker.edu/login?url={targeturl}.
    • Chicago/Turabian: Humanities:
      Conceição, Aparecido Almeida, Clemente Batista Soares Neto, José Antônio de Aquino Ribeiro, Felix Gonçalves de Siqueira, Robert Neil Gerard Miller, and Simone Mendonça. “Development of an RP-UHPLC-PDA Method for Quantification of Free Gossypol in Cottonseed Cake and Fungal-Treated Cottonseed Cake.” PLoS ONE 13, no. 5 (May 23, 2018): 1. http://ezproxy.parker.edu/login?url={targeturl}.
    • Vancouver/ICMJE:
      Conceição AA, Soares Neto CB, Ribeiro JA de A, Siqueira FG de, Miller RNG, Mendonça S. Development of an RP-UHPLC-PDA method for quantification of free gossypol in cottonseed cake and fungal-treated cottonseed cake. PLoS ONE [Internet]. 2018 May 23 [cited 2019 Aug 24];13(5):1. Available from: http://ezproxy.parker.edu/login?url={targeturl}