MagA expression attenuates iron export activity in undifferentiated multipotent P19 cells.

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  • Additional Information
    • Publication Year:
      2019
    • Author-Supplied Keywords:
      Biological cultures
      Biology and life sciences
      Cell biology
      Cell lines
      Cell membranes
      Cellular structures and organelles
      Chemical compounds
      Chemical elements
      Chemistry
      Diagnostic medicine
      Diagnostic radiology
      Imaging techniques
      Intracellular membranes
      Iron
      Magnetic resonance imaging
      Medicine and health sciences
      Membrane proteins
      Nitrates
      NMR relaxation
      NMR spectroscopy
      P19 cells
      Physical sciences
      Radiology and imaging
      Research and analysis methods
      Research Article
      Spectrum analysis techniques
    • Abstract:
      Magnetic resonance imaging (MRI) is a non-invasive imaging modality used in longitudinal cell tracking. Previous studies suggest that MagA, a putative iron transport protein from magnetotactic bacteria, is a useful gene-based magnetic resonance contrast agent. Hemagglutinin-tagged MagA was stably expressed in undifferentiated embryonic mouse teratocarcinoma, multipotent P19 cells to provide a suitable model for tracking these cells during differentiation. Western blot and immunocytochemistry confirmed the expression and membrane localization of MagA in P19 cells. Surprisingly, elemental iron analysis using inductively-coupled plasma mass spectrometry revealed significant iron uptake in both parental and MagA-expressing P19 cells, cultured in the presence of iron-supplemented medium. Withdrawal of this extracellular iron supplement revealed unexpected iron export activity in P19 cells, which MagA expression attenuated. The influence of iron supplementation on parental and MagA-expressing cells was not reflected by longitudinal relaxation rates. Measurement of transverse relaxation rates (R2* and R2) reflected changes in total cellular iron content but did not clearly distinguish MagA-expressing cells from the parental cell type, despite significant differences in the uptake and retention of total cellular iron. Unlike other cell types, the reversible component R2′ (R2* ‒ R2) provided only a moderately strong correlation to amount of cellular iron, normalized to amount of protein. This is the first report to characterize MagA expression in a previously unrecognized iron exporting cell type. The interplay between contrast gene expression and systemic iron metabolism substantiates the potential for diverting cellular iron toward the formation of a novel iron compartment, however rudimentary when using a single magnetotactic bacterial gene expression system like magA. Since relatively few mammalian cells export iron, the P19 cell line provides a tractable model of ferroportin activity, suitable for magnetic resonance analysis of key iron-handling activities and their influence on gene-based MRI contrast. [ABSTRACT FROM AUTHOR]
    • Abstract:
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    • ISSN:
      19326203
    • Accession Number:
      136835871
  • Citations
    • ABNT:
      LIU, L. et al. MagA expression attenuates iron export activity in undifferentiated multipotent P19 cells. PLoS ONE, [s. l.], v. 14, n. 6, p. 1, 2019. Disponível em: . Acesso em: 24 ago. 2019.
    • AMA:
      Liu L, Alizadeh K, Donnelly SC, et al. MagA expression attenuates iron export activity in undifferentiated multipotent P19 cells. PLoS ONE. 2019;14(6):1. http://ezproxy.parker.edu/login?url={targeturl}. Accessed August 24, 2019.
    • APA:
      Liu, L., Alizadeh, K., Donnelly, S. C., Dassanayake, P., Hou, T. T., McGirr, R., … Goldhawk, D. E. (2019). MagA expression attenuates iron export activity in undifferentiated multipotent P19 cells. PLoS ONE, 14(6), 1. Retrieved from http://ezproxy.parker.edu/login?url={targeturl}
    • Chicago/Turabian: Author-Date:
      Liu, Linshan, Kobra Alizadeh, Sarah C. Donnelly, Praveen Dassanayake, Tian Tian Hou, Rebecca McGirr, R. Terry Thompson, et al. 2019. “MagA Expression Attenuates Iron Export Activity in Undifferentiated Multipotent P19 Cells.” PLoS ONE 14 (6): 1. http://ezproxy.parker.edu/login?url={targeturl}.
    • Harvard:
      Liu, L. et al. (2019) ‘MagA expression attenuates iron export activity in undifferentiated multipotent P19 cells’, PLoS ONE, 14(6), p. 1. Available at: http://ezproxy.parker.edu/login?url={targeturl} (Accessed: 24 August 2019).
    • Harvard: Australian:
      Liu, L, Alizadeh, K, Donnelly, SC, Dassanayake, P, Hou, TT, McGirr, R, Thompson, RT, Prato, FS, Gelman, N, Hoffman, L & Goldhawk, DE 2019, ‘MagA expression attenuates iron export activity in undifferentiated multipotent P19 cells’, PLoS ONE, vol. 14, no. 6, p. 1, viewed 24 August 2019, .
    • MLA:
      Liu, Linshan, et al. “MagA Expression Attenuates Iron Export Activity in Undifferentiated Multipotent P19 Cells.” PLoS ONE, vol. 14, no. 6, June 2019, p. 1. EBSCOhost, ezproxy.parker.edu/login?url={targeturl}.
    • Chicago/Turabian: Humanities:
      Liu, Linshan, Kobra Alizadeh, Sarah C. Donnelly, Praveen Dassanayake, Tian Tian Hou, Rebecca McGirr, R. Terry Thompson, et al. “MagA Expression Attenuates Iron Export Activity in Undifferentiated Multipotent P19 Cells.” PLoS ONE 14, no. 6 (June 6, 2019): 1. http://ezproxy.parker.edu/login?url={targeturl}.
    • Vancouver/ICMJE:
      Liu L, Alizadeh K, Donnelly SC, Dassanayake P, Hou TT, McGirr R, et al. MagA expression attenuates iron export activity in undifferentiated multipotent P19 cells. PLoS ONE [Internet]. 2019 Jun 6 [cited 2019 Aug 24];14(6):1. Available from: http://ezproxy.parker.edu/login?url={targeturl}