Metabolites of Mangrove Sediment Bacteria from Semarang and Karimunjawa as Anti-Fungal and Antibacterial.

Pathogenic bacteria are microorganisms that cause disease in humans because bacteria grow cosmopolitan with human life, both on land and underwater. This research aimed to obtain mangrove sediment bacteria that have the potential as antibacterial and antifungal bacteria from mangrove sediments in Semarang and Karimunjawa waters. Mangrove sediment bacteria that have the potential as antibacterial and antifungal bacteria from Semarang and Karimunjawa waters, as well as identification of their compounds using the GCMS method. Sediment samples were collected from Semarang and Karimunjawa, and bacterial screening and isolation were then carried out. Bacterial isolates were tested with pathogenic fungi Malassezia furfur and Candida albicans. As well as the pathogenic bacteria Staphylococcus aureus, Escherichia coli, and Staphylococcus typhi. Then, the potential bacteria were identified by the molecular DNA method and analyzed by the GC-MS method to determine the secondary metabolite compounds that act as antifungal and antibacterial. The results showed that the bacterial isolation of mangrove sediments from Karimunjawa and Semarang that had antibacterial activity against pathogenic bacteria E. coli, S. aureus, and S. typhi were 3 isolates, namely SKK 1 MA, SKS 2 ISP, and SKS 4 ISP. The 3 isolates also had antifungal activity against the fungal pathogens Malassezia furfur and Candida albicans. The molecular identification showed that the 3 potential isolates, namely SKK 1 MA, SKS 2 ISP, and SKS 4 ISP, were Bacillus sp., Bacillus cereus, and Alcaligenes faecalis, respectively. Results of the analysis of the content of bacteria as antifungal and antibacterial showed that the bacteria were dominated by the content of unsaturated fatty acids (omega 9) and saturated fatty acids. [ABSTRACT FROM AUTHOR]

Copyright of Trends in Sciences is the property of Walailak Journal of Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)