Fertilization in C. elegans requires an intact C-terminal RING finger in sperm protein SPE-42.

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  • Additional Information
    • Publication Year:
      2011
    • NAICS/Industry Codes:
      NAICS/Industry Codes 416210 Metal service centres
    • Abstract:
      Background: The C. elegans sperm protein SPE-42, a membrane protein of unknown structure and molecular function, is required for fertilization. Sperm from worms with spe-42 mutations appear normal but are unable to fertilize eggs. Sequence analysis revealed the presence of 8 conserved cysteine residues in the C-terminal cytoplasmic domain of this protein suggesting these residues form a zinc-coordinating RING finger structure. Results: We made an in silico structural model of the SPE-42 RING finger domain based on primary sequence analysis and previously reported RING structures. To test the model, we created spe-42 transgenes coding for mutations in each of the 8 cysteine residues predicted to coordinate Zn++ ions in the RING finger motif. Transgenes were crossed into a spe-42 null background and protein function was measured by counting progeny. We found that all 8 cysteines are required for protein function. We also showed that sequence differences between the C-terminal 29 and 30 amino acids in C. elegans and C. briggsae SPE-42 following the RING finger domain are not responsible for the failure of the C. briggsae SPE-42 homolog to rescue C. elegans spe-42 mutants. Conclusions: The results suggest that a bona fide RING domain is present at the C-terminus of the SPE-42 protein and that this motif is required for sperm-egg interactions during C. elegans fertilization. Our structural model of the RING domain provides a starting point for further structure-function analysis of this critical region of the protein. The C-terminal domain swap experiment suggests that the incompatibility between the C. elegans and C. briggsae SPE-42 proteins is caused by small amino acid differences outside the C-terminal domain. [ABSTRACT FROM AUTHOR]
    • Abstract:
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    • ISSN:
      1471213X
    • Accession Number:
      59748255
  • Citations
    • ABNT:
      WILSON, L. D. et al. Fertilization in C. elegans requires an intact C-terminal RING finger in sperm protein SPE-42. BMC Developmental Biology, [s. l.], v. 11, n. 1, p. 10, 2011. Disponível em: http://widgets.ebscohost.com/prod/customlink/proxify/proxify.php?count=1&encode=0&proxy=&find_1=&replace_1=&target=http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=59748255&authtype=sso&custid=s5834912. Acesso em: 8 jul. 2020.
    • AMA:
      Wilson LD, Sackett JM, Mieczkowski BD, et al. Fertilization in C. elegans requires an intact C-terminal RING finger in sperm protein SPE-42. BMC Developmental Biology. 2011;11(1):10. http://widgets.ebscohost.com/prod/customlink/proxify/proxify.php?count=1&encode=0&proxy=&find_1=&replace_1=&target=http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=59748255&authtype=sso&custid=s5834912. Accessed July 8, 2020.
    • AMA11:
      Wilson LD, Sackett JM, Mieczkowski BD, et al. Fertilization in C. elegans requires an intact C-terminal RING finger in sperm protein SPE-42. BMC Developmental Biology. 2011;11(1):10. Accessed July 8, 2020. http://widgets.ebscohost.com/prod/customlink/proxify/proxify.php?count=1&encode=0&proxy=&find_1=&replace_1=&target=http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=59748255&authtype=sso&custid=s5834912
    • APA:
      Wilson, L. D., Sackett, J. M., Mieczkowski, B. D., Richie, A. L., Thoemke, K., Rumbley, J. N., & Kroft, T. L. (2011). Fertilization in C. elegans requires an intact C-terminal RING finger in sperm protein SPE-42. BMC Developmental Biology, 11(1), 10.
    • Chicago/Turabian: Author-Date:
      Wilson, Luke D., Jacqueline M. Sackett, Bryce D. Mieczkowski, Abigail L. Richie, Kara Thoemke, Jon N. Rumbley, and Tim L. Kroft. 2011. “Fertilization in C. Elegans Requires an Intact C-Terminal RING Finger in Sperm Protein SPE-42.” BMC Developmental Biology 11 (1): 10. http://widgets.ebscohost.com/prod/customlink/proxify/proxify.php?count=1&encode=0&proxy=&find_1=&replace_1=&target=http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=59748255&authtype=sso&custid=s5834912.
    • Harvard:
      Wilson, L. D. et al. (2011) ‘Fertilization in C. elegans requires an intact C-terminal RING finger in sperm protein SPE-42’, BMC Developmental Biology, 11(1), p. 10. Available at: http://widgets.ebscohost.com/prod/customlink/proxify/proxify.php?count=1&encode=0&proxy=&find_1=&replace_1=&target=http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=59748255&authtype=sso&custid=s5834912 (Accessed: 8 July 2020).
    • Harvard: Australian:
      Wilson, LD, Sackett, JM, Mieczkowski, BD, Richie, AL, Thoemke, K, Rumbley, JN & Kroft, TL 2011, ‘Fertilization in C. elegans requires an intact C-terminal RING finger in sperm protein SPE-42’, BMC Developmental Biology, vol. 11, no. 1, p. 10, viewed 8 July 2020, .
    • MLA:
      Wilson, Luke D., et al. “Fertilization in C. Elegans Requires an Intact C-Terminal RING Finger in Sperm Protein SPE-42.” BMC Developmental Biology, vol. 11, no. 1, Jan. 2011, p. 10. EBSCOhost, widgets.ebscohost.com/prod/customlink/proxify/proxify.php?count=1&encode=0&proxy=&find_1=&replace_1=&target=http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=59748255&authtype=sso&custid=s5834912.
    • Chicago/Turabian: Humanities:
      Wilson, Luke D., Jacqueline M. Sackett, Bryce D. Mieczkowski, Abigail L. Richie, Kara Thoemke, Jon N. Rumbley, and Tim L. Kroft. “Fertilization in C. Elegans Requires an Intact C-Terminal RING Finger in Sperm Protein SPE-42.” BMC Developmental Biology 11, no. 1 (January 2011): 10. http://widgets.ebscohost.com/prod/customlink/proxify/proxify.php?count=1&encode=0&proxy=&find_1=&replace_1=&target=http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=59748255&authtype=sso&custid=s5834912.
    • Vancouver/ICMJE:
      Wilson LD, Sackett JM, Mieczkowski BD, Richie AL, Thoemke K, Rumbley JN, et al. Fertilization in C. elegans requires an intact C-terminal RING finger in sperm protein SPE-42. BMC Developmental Biology [Internet]. 2011 Jan [cited 2020 Jul 8];11(1):10. Available from: http://widgets.ebscohost.com/prod/customlink/proxify/proxify.php?count=1&encode=0&proxy=&find_1=&replace_1=&target=http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edb&AN=59748255&authtype=sso&custid=s5834912