Daidzein and the daidzein metabolite, equol, enhance adipocyte differentiation and PPARγ transcriptional activity

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  • Additional Information
    • Affiliation:
      a Department of Nutrition, University of Massachusetts, Amherst, MA 01003, USA
      b Department of Nutrition and Animal Science, Southern Illinois University, Carbondale, IL 62901, USA
      c Department of Animal Science and UT Obesity Research Center, The University of Tennessee, Knoxville, TN, 37996, USA
      d Department of Food Science and Nutrition, University of Florida, FL, 32611, USA
    • Keywords:
      Daidzein
      Equol
      PPARγ
      Adipocyte differentiation
      Insulin sensitivity
      GLUT4
    • Abstract:
      Dietary soy isoflavones have been shown to favorably alter the metabolic phenotypes associated with Type 2 diabetes. However, the identification of direct targets and the underlying molecular mechanisms by which soy isoflaovones exert antidiabetic effects remain elusive. Since the insulin-sensitizing effects of thiazolidinediones, antidiabetic drugs, are mediated through activation of peroxisome proliferators-activated receptor gamma (PPARγ), we examined the effects of daidzein and the daidzein metabolite, equol, on adipocyte differentiation and PPARγ activation. In 3T3-L1 cells, daidzein enhanced adipocyte differentiation and PPARγ expression in a dose-dependent manner. Daidzein also dose-dependently increased insulin-stimulated glucose uptake and the relative abundance of insulin-responsive glucose transporter 4 (GLUT4) and insulin receptor substrate 1 (IRS-1) mRNA. In C3H10T1/2 cells, both daidzein and equol at 1 μmol/L and higher significantly increased adipocyte differentiation and insulin-stimulated glucose uptake. Furthermore, daidzein and equol up-regulated PPARγ-mediated transcriptional activity, and daidzein restored the PPARγ antagonist-induced inhibition of aP2 and GLUT4 mRNA levels. Our results indicate that daidzein enhances insulin-stimulated glucose uptake in adipocytes by increasing the expression of GLUT4 and IRS-1 via the activation of PPARγ. These data further support the recent findings that favorable effects of dietary soy isoflavones may be attributable to daidzein and its metabolite equol.
    • ISSN:
      0955-2863
    • Accession Number:
      10.1016/j.jnutbio.2009.06.012
    • Accession Number:
      S0955286309001363
    • Copyright:
      Published by Elsevier Inc.
  • Citations
    • ABNT:
      CHO, K. W. et al. Daidzein and the daidzein metabolite, equol, enhance adipocyte differentiation and PPARγ transcriptional activity. The Journal of Nutritional Biochemistry, [s. l.], v. 21, n. 9, p. 841–847, 2010. DOI 10.1016/j.jnutbio.2009.06.012. Disponível em: http://widgets.ebscohost.com/prod/customlink/proxify/proxify.php?count=1&encode=0&proxy=&find_1=&replace_1=&target=http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edselp&AN=S0955286309001363&authtype=sso&custid=s5834912. Acesso em: 27 jan. 2020.
    • AMA:
      Cho KW, Lee O-H, Banz WJ, Moustaid-Moussa N, Shay NF, Kim Y-C. Daidzein and the daidzein metabolite, equol, enhance adipocyte differentiation and PPARγ transcriptional activity. The Journal of Nutritional Biochemistry. 2010;21(9):841-847. doi:10.1016/j.jnutbio.2009.06.012.
    • APA:
      Cho, K. W., Lee, O.-H., Banz, W. J., Moustaid-Moussa, N., Shay, N. F., & Kim, Y.-C. (2010). Daidzein and the daidzein metabolite, equol, enhance adipocyte differentiation and PPARγ transcriptional activity. The Journal of Nutritional Biochemistry, 21(9), 841–847. https://doi.org/10.1016/j.jnutbio.2009.06.012
    • Chicago/Turabian: Author-Date:
      Cho, Kae Won, Ok-Hwan Lee, William J. Banz, Naima Moustaid-Moussa, Neil F. Shay, and Young-Cheul Kim. 2010. “Daidzein and the Daidzein Metabolite, Equol, Enhance Adipocyte Differentiation and PPARγ Transcriptional Activity.” The Journal of Nutritional Biochemistry 21 (9): 841–47. doi:10.1016/j.jnutbio.2009.06.012.
    • Harvard:
      Cho, K. W. et al. (2010) ‘Daidzein and the daidzein metabolite, equol, enhance adipocyte differentiation and PPARγ transcriptional activity’, The Journal of Nutritional Biochemistry, 21(9), pp. 841–847. doi: 10.1016/j.jnutbio.2009.06.012.
    • Harvard: Australian:
      Cho, KW, Lee, O-H, Banz, WJ, Moustaid-Moussa, N, Shay, NF & Kim, Y-C 2010, ‘Daidzein and the daidzein metabolite, equol, enhance adipocyte differentiation and PPARγ transcriptional activity’, The Journal of Nutritional Biochemistry, vol. 21, no. 9, pp. 841–847, viewed 27 January 2020, .
    • MLA:
      Cho, Kae Won, et al. “Daidzein and the Daidzein Metabolite, Equol, Enhance Adipocyte Differentiation and PPARγ Transcriptional Activity.” The Journal of Nutritional Biochemistry, vol. 21, no. 9, Jan. 2010, pp. 841–847. EBSCOhost, doi:10.1016/j.jnutbio.2009.06.012.
    • Chicago/Turabian: Humanities:
      Cho, Kae Won, Ok-Hwan Lee, William J. Banz, Naima Moustaid-Moussa, Neil F. Shay, and Young-Cheul Kim. “Daidzein and the Daidzein Metabolite, Equol, Enhance Adipocyte Differentiation and PPARγ Transcriptional Activity.” The Journal of Nutritional Biochemistry 21, no. 9 (January 1, 2010): 841–47. doi:10.1016/j.jnutbio.2009.06.012.
    • Vancouver/ICMJE:
      Cho KW, Lee O-H, Banz WJ, Moustaid-Moussa N, Shay NF, Kim Y-C. Daidzein and the daidzein metabolite, equol, enhance adipocyte differentiation and PPARγ transcriptional activity. The Journal of Nutritional Biochemistry [Internet]. 2010 Jan 1 [cited 2020 Jan 27];21(9):841–7. Available from: http://widgets.ebscohost.com/prod/customlink/proxify/proxify.php?count=1&encode=0&proxy=&find_1=&replace_1=&target=http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edselp&AN=S0955286309001363&authtype=sso&custid=s5834912