Exposure to a Complex Cocktail of Environmental Endocrine-Disrupting Compounds Disturbs the Kisspeptin/GPR54 System in Ovine Hypothalamus and Pituitary Gland.

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  • Additional Information
    • Subject Terms:
    • Author-Supplied Keywords:
      environmental chemicals
      GPR54
      hypothalamus
      kisspeptin
      pituitary
      prenatal exposure
      sheep
    • Abstract:
      Background: Ubiquitous environmental chemicals, including endocrine-disrupting chemicals (EDCs), are associated with declining human reproductive health, as well as an increasing incidence of cancers of the reproductive system. Verifying such links requires animal models exposed to "reallife," environmentally relevant concentrations/mixtures of EDC, particularly in utero, when sensitivity to EDC exposure is maximal. Objectives: We evaluated the effects of maternal exposure to a pollutant cocktail (sewage sludge) on the ovine fetal reproductive neuroendocrine axes, particularly the kisspeptin (KiSS-1)/GPR54 (G-protein-coupled receptor 54) system. Methods: KiSS-1, GPR54, and ERα (estrogen receptor α) mRNA expression was quantified in control (C) and treated (T) maternal and fetal (110-day) hypothalami and pituitary glands using semiquantitative reverse transcription polymerase chain reaction, and colocalization of kisspeptin with LHβ (luteinizing hormone β) and ERα in C and T fetal pituitary glands quantified using duallabeling immunohistochemistry. Results: Fetuses exposed in utero to the EDC mixture showed reduced KiSS-1 mRNA expression across three hypothalamic regions examined (rostral, mid, and caudal) and had fewer kisspetin immunopositive cells colocalized with both LHβ and ERα in the pituitary gland. In contrast, treatment had no effect on parameters measured in the adult ewe hypothalamus or pituitary. Conclusions: This study demonstrates that the developing fetus is sensitive to real-world mixtures of environmental chemicals, which cause significant neuroendocrine alterations. The important role of kisspeptin/GPR54 in regulating puberty and adult reproduction means that in utero disruption of this system is likely to have long-term consequences in adulthood and represents a novel, additional pathway through which environmental chemicals perturb human reproduction. [ABSTRACT FROM AUTHOR]
    • Abstract:
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    • ISSN:
      0091-6765
    • Accession Number:
      10.1289/ehp.0900699
    • Accession Number:
      44707398
  • Citations
    • ABNT:
      BELLINGHAM, M. et al. Exposure to a Complex Cocktail of Environmental Endocrine-Disrupting Compounds Disturbs the Kisspeptin/GPR54 System in Ovine Hypothalamus and Pituitary Gland. Environmental Health Perspectives, [s. l.], v. 117, n. 10, p. 1556–1562, 2009. Disponível em: . Acesso em: 24 ago. 2019.
    • AMA:
      Bellingham M, Fowler PA, Amezaga MR, et al. Exposure to a Complex Cocktail of Environmental Endocrine-Disrupting Compounds Disturbs the Kisspeptin/GPR54 System in Ovine Hypothalamus and Pituitary Gland. Environmental Health Perspectives. 2009;117(10):1556-1562. doi:10.1289/ehp.0900699.
    • APA:
      Bellingham, M., Fowler, P. A., Amezaga, M. R., Rhind, S. M., Cotinot, C., Mandon-Pepin, B., … Evans, N. P. (2009). Exposure to a Complex Cocktail of Environmental Endocrine-Disrupting Compounds Disturbs the Kisspeptin/GPR54 System in Ovine Hypothalamus and Pituitary Gland. Environmental Health Perspectives, 117(10), 1556–1562. https://doi.org/10.1289/ehp.0900699
    • Chicago/Turabian: Author-Date:
      Bellingham, Michelle, Paul A. Fowler, Maria R. Amezaga, Stewart M. Rhind, Corinne Cotinot, Beatrice Mandon-Pepin, Richard M. Sharpe, and Neil P. Evans. 2009. “Exposure to a Complex Cocktail of Environmental Endocrine-Disrupting Compounds Disturbs the Kisspeptin/GPR54 System in Ovine Hypothalamus and Pituitary Gland.” Environmental Health Perspectives 117 (10): 1556–62. doi:10.1289/ehp.0900699.
    • Harvard:
      Bellingham, M. et al. (2009) ‘Exposure to a Complex Cocktail of Environmental Endocrine-Disrupting Compounds Disturbs the Kisspeptin/GPR54 System in Ovine Hypothalamus and Pituitary Gland’, Environmental Health Perspectives, 117(10), pp. 1556–1562. doi: 10.1289/ehp.0900699.
    • Harvard: Australian:
      Bellingham, M, Fowler, PA, Amezaga, MR, Rhind, SM, Cotinot, C, Mandon-Pepin, B, Sharpe, RM & Evans, NP 2009, ‘Exposure to a Complex Cocktail of Environmental Endocrine-Disrupting Compounds Disturbs the Kisspeptin/GPR54 System in Ovine Hypothalamus and Pituitary Gland’, Environmental Health Perspectives, vol. 117, no. 10, pp. 1556–1562, viewed 24 August 2019, .
    • MLA:
      Bellingham, Michelle, et al. “Exposure to a Complex Cocktail of Environmental Endocrine-Disrupting Compounds Disturbs the Kisspeptin/GPR54 System in Ovine Hypothalamus and Pituitary Gland.” Environmental Health Perspectives, vol. 117, no. 10, Oct. 2009, pp. 1556–1562. EBSCOhost, doi:10.1289/ehp.0900699.
    • Chicago/Turabian: Humanities:
      Bellingham, Michelle, Paul A. Fowler, Maria R. Amezaga, Stewart M. Rhind, Corinne Cotinot, Beatrice Mandon-Pepin, Richard M. Sharpe, and Neil P. Evans. “Exposure to a Complex Cocktail of Environmental Endocrine-Disrupting Compounds Disturbs the Kisspeptin/GPR54 System in Ovine Hypothalamus and Pituitary Gland.” Environmental Health Perspectives 117, no. 10 (October 2009): 1556–62. doi:10.1289/ehp.0900699.
    • Vancouver/ICMJE:
      Bellingham M, Fowler PA, Amezaga MR, Rhind SM, Cotinot C, Mandon-Pepin B, et al. Exposure to a Complex Cocktail of Environmental Endocrine-Disrupting Compounds Disturbs the Kisspeptin/GPR54 System in Ovine Hypothalamus and Pituitary Gland. Environmental Health Perspectives [Internet]. 2009 Oct [cited 2019 Aug 24];117(10):1556–62. Available from: http://ezproxy.parker.edu/login?url={targeturl}