Neuron-restricted expression of the rat gonadotropin-releasing hormone gene is conferred by a cell-specific protein complex that binds repeated CAATT elements.

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  • Additional Information
    • Source:
      Publisher: Endocrine Society Country of Publication: United States NLM ID: 8801431 Publication Model: Print Cited Medium: Print ISSN: 0888-8809 (Print) Linking ISSN: 08888809 NLM ISO Abbreviation: Mol. Endocrinol. Subsets: MEDLINE
    • Publication Information:
      Publication: Chevy Chase, MD : Endocrine Society
      Original Publication: [Baltimore, MD : Endocrine Society, c1987-
    • Subject Terms:
    • Abstract:
      GnRH gene expression is restricted to a tiny population of neurons scattered throughout the mediobasal hypothalamus. The combination of a 300-bp enhancer and the 173-bp promoter from the rat GnRH gene can confer this narrow specificity in transgenic mice and in transfections of hypothalamic GT1-7 cells. In the present study, we identify repeated CAATT elements in the 3' region of the rat GnRH enhancer that bind a tissue-restricted protein complex and play a significant role in cell-restricted expression of the GnRH gene. Deletions of multiple repeats demonstrate their importance in transcriptional activity. In fact, even mutation of a single repeat reduces expression. This reduction can be compensated by the conserved GnRH promoter, which also contains such elements and binds this protein complex. In Southwestern analysis, three proteins from GT1-7 nuclear extract bind to the CAATT element, and these proteins are not found in NIH3T3 cells. This cell-specific protein complex has properties of the Q50 homeodomain family of transcription factors and binds to as many as seven binding sites in the enhancer and promoter to play a key role in GnRH gene expression in the hypothalamus.
    • References:
      Development. 1995 Nov;121(11):3877-88. (PMID: 8582296)
      Development. 1996 Jan;122(1):243-52. (PMID: 8565836)
      Mol Endocrinol. 1995 Jul;9(7):848-59. (PMID: 7476968)
      Mol Cell Biol. 1996 Jul;16(7):3596-605. (PMID: 8668176)
      Nat Genet. 1996 Oct;14(2):218-22. (PMID: 8841200)
      Mech Dev. 1996 Aug;58(1-2):165-78. (PMID: 8887325)
      Mol Endocrinol. 1998 Mar;12(3):364-77. (PMID: 9514154)
      Mol Endocrinol. 1998 Apr;12(4):469-81. (PMID: 9544983)
      Mol Cell Endocrinol. 1998 May 25;140(1-2):157-61. (PMID: 9722184)
      J Neurobiol. 1998 Sep 15;36(4):559-71. (PMID: 9740027)
      Curr Opin Genet Dev. 1998 Oct;8(5):552-9. (PMID: 9794820)
      J Biol Chem. 1999 Feb 26;274(9):6020-6. (PMID: 10026229)
      Annu Rev Biophys Biomol Struct. 1999;28:29-56. (PMID: 10410794)
      Mol Endocrinol. 2000 Feb;14(2):212-28. (PMID: 10674395)
      Mol Endocrinol. 2000 Aug;14(8):1246-56. (PMID: 10935548)
      Mol Endocrinol. 2000 Sep;14(9):1509-22. (PMID: 10976927)
      Endocrinology. 2002 Apr;143(4):1404-12. (PMID: 11897697)
      Genes Dev. 1987 Dec;1(10):1147-60. (PMID: 2828167)
      Cell. 1988 Sep 23;54(7):1081-90. (PMID: 3046753)
      Proc Natl Acad Sci U S A. 1989 Oct;86(20):8132-6. (PMID: 2682637)
      Neuron. 1990 Jul;5(1):1-10. (PMID: 2196069)
      Proc Natl Acad Sci U S A. 1991 Apr 15;88(8):3402-6. (PMID: 2014260)
      Biochemistry. 1991 Dec 3;30(48):11357-67. (PMID: 1742275)
      Mol Cell Biol. 1992 May;12(5):2143-53. (PMID: 1373809)
      Endocr Rev. 1992 Nov;13(4):623-34. (PMID: 1459046)
      Mol Cell Biol. 1993 Apr;13(4):2235-46. (PMID: 8455608)
      Mol Cell Biol. 1994 Aug;14(8):5592-602. (PMID: 7518566)
      Dev Biol. 1994 Aug;164(2):361-73. (PMID: 8045339)
      Science. 1995 Aug 4;269(5224):679-82. (PMID: 7624797)
      Mol Endocrinol. 1995 Apr;9(4):467-77. (PMID: 7659090)
      Mol Cell Biol. 1995 Nov;15(11):6169-77. (PMID: 7565769)
      Genes Dev. 1995 Nov 1;9(21):2646-58. (PMID: 7590242)
    • Grant Information:
      R01 HD072754 United States HD NICHD NIH HHS; T32 DA007315 United States DA NIDA NIH HHS; T-32-DA-07315 United States DA NIDA NIH HHS; DK-44838 United States DK NIDDK NIH HHS; T32 AG000216 United States AG NIA NIH HHS; T-32-AG-00216 United States AG NIA NIH HHS; R01 DK044838 United States DK NIDDK NIH HHS; P50 HD012303 United States HD NICHD NIH HHS; R01 DK044838-15 United States DK NIDDK NIH HHS
    • Accession Number:
      0 (CCAAT-Enhancer-Binding Proteins)
      0 (DNA-Binding Proteins)
      0 (Recombinant Proteins)
      33515-09-2 (Gonadotropin-Releasing Hormone)
    • Publication Date:
      Date Created: 20021031 Date Completed: 20030618 Latest Revision: 20181113
    • Publication Date:
      20181210
    • Accession Number:
      12403831
  • Citations
    • ABNT:
      KELLEY, C. G. et al. Neuron-restricted expression of the rat gonadotropin-releasing hormone gene is conferred by a cell-specific protein complex that binds repeated CAATT elements. Molecular Endocrinology (Baltimore, Md.), [s. l.], v. 16, n. 11, p. 2413–2425, 2002. Disponível em: http://widgets.ebscohost.com/prod/customlink/proxify/proxify.php?count=1&encode=0&proxy=&find_1=&replace_1=&target=http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=mdc&AN=12403831&authtype=sso&custid=s5834912. Acesso em: 19 fev. 2020.
    • AMA:
      Kelley CG, Givens ML, Rave-Harel N, Nelson SB, Anderson S, Mellon PL. Neuron-restricted expression of the rat gonadotropin-releasing hormone gene is conferred by a cell-specific protein complex that binds repeated CAATT elements. Molecular Endocrinology (Baltimore, Md). 2002;16(11):2413-2425. http://widgets.ebscohost.com/prod/customlink/proxify/proxify.php?count=1&encode=0&proxy=&find_1=&replace_1=&target=http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=mdc&AN=12403831&authtype=sso&custid=s5834912. Accessed February 19, 2020.
    • APA:
      Kelley, C. G., Givens, M. L., Rave-Harel, N., Nelson, S. B., Anderson, S., & Mellon, P. L. (2002). Neuron-restricted expression of the rat gonadotropin-releasing hormone gene is conferred by a cell-specific protein complex that binds repeated CAATT elements. Molecular Endocrinology (Baltimore, Md.), 16(11), 2413–2425.
    • Chicago/Turabian: Author-Date:
      Kelley, Carolyn G, Marjory L Givens, Naama Rave-Harel, Shelley B Nelson, Scott Anderson, and Pamela L Mellon. 2002. “Neuron-Restricted Expression of the Rat Gonadotropin-Releasing Hormone Gene Is Conferred by a Cell-Specific Protein Complex That Binds Repeated CAATT Elements.” Molecular Endocrinology (Baltimore, Md.) 16 (11): 2413–25. http://widgets.ebscohost.com/prod/customlink/proxify/proxify.php?count=1&encode=0&proxy=&find_1=&replace_1=&target=http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=mdc&AN=12403831&authtype=sso&custid=s5834912.
    • Harvard:
      Kelley, C. G. et al. (2002) ‘Neuron-restricted expression of the rat gonadotropin-releasing hormone gene is conferred by a cell-specific protein complex that binds repeated CAATT elements’, Molecular Endocrinology (Baltimore, Md.), 16(11), pp. 2413–2425. Available at: http://widgets.ebscohost.com/prod/customlink/proxify/proxify.php?count=1&encode=0&proxy=&find_1=&replace_1=&target=http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=mdc&AN=12403831&authtype=sso&custid=s5834912 (Accessed: 19 February 2020).
    • Harvard: Australian:
      Kelley, CG, Givens, ML, Rave-Harel, N, Nelson, SB, Anderson, S & Mellon, PL 2002, ‘Neuron-restricted expression of the rat gonadotropin-releasing hormone gene is conferred by a cell-specific protein complex that binds repeated CAATT elements’, Molecular Endocrinology (Baltimore, Md.), vol. 16, no. 11, pp. 2413–2425, viewed 19 February 2020, .
    • MLA:
      Kelley, Carolyn G., et al. “Neuron-Restricted Expression of the Rat Gonadotropin-Releasing Hormone Gene Is Conferred by a Cell-Specific Protein Complex That Binds Repeated CAATT Elements.” Molecular Endocrinology (Baltimore, Md.), vol. 16, no. 11, Nov. 2002, pp. 2413–2425. EBSCOhost, widgets.ebscohost.com/prod/customlink/proxify/proxify.php?count=1&encode=0&proxy=&find_1=&replace_1=&target=http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=mdc&AN=12403831&authtype=sso&custid=s5834912.
    • Chicago/Turabian: Humanities:
      Kelley, Carolyn G, Marjory L Givens, Naama Rave-Harel, Shelley B Nelson, Scott Anderson, and Pamela L Mellon. “Neuron-Restricted Expression of the Rat Gonadotropin-Releasing Hormone Gene Is Conferred by a Cell-Specific Protein Complex That Binds Repeated CAATT Elements.” Molecular Endocrinology (Baltimore, Md.) 16, no. 11 (November 2002): 2413–25. http://widgets.ebscohost.com/prod/customlink/proxify/proxify.php?count=1&encode=0&proxy=&find_1=&replace_1=&target=http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=mdc&AN=12403831&authtype=sso&custid=s5834912.
    • Vancouver/ICMJE:
      Kelley CG, Givens ML, Rave-Harel N, Nelson SB, Anderson S, Mellon PL. Neuron-restricted expression of the rat gonadotropin-releasing hormone gene is conferred by a cell-specific protein complex that binds repeated CAATT elements. Molecular Endocrinology (Baltimore, Md) [Internet]. 2002 Nov [cited 2020 Feb 19];16(11):2413–25. Available from: http://widgets.ebscohost.com/prod/customlink/proxify/proxify.php?count=1&encode=0&proxy=&find_1=&replace_1=&target=http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=mdc&AN=12403831&authtype=sso&custid=s5834912